目的探讨Survivin基因在非小细胞肺癌(NSCLC)组织中的表达及其与临床病理因素的关系。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测80例非小细胞肺癌组织以及60例癌旁肺组织中Survivin基因mRNA的表达。结果 Survivin在NSCLC组织中阳性表达率为67.75%(55/80),在NSCLC癌旁组织中阳性表达为41.6%(25/60),肺癌组织和癌旁组织中Survivin基因表达差异有统计学意义(P<0.05),Survivin在不同的临床分期、有无淋巴结转移中的表达差异有统计学意义(P<0.05)。结论检测肺癌组织中Survivin的表达,可作为判断肺癌转移和分期以及预后的指标。
Objective To explore the expression and relationship with clinical pathological elements of survivin in patients with non-small cell lung cancer ( NSCLC) . Methods Messenger RNA expression of survivin gene was examined in 80 tumor tissues and 60 adjacent tissue samples using semi-quantitative RT-PCR. Results The positive expression rate of survivin in tissues of non-small cell lung cancer was 67. 75% ( 55 /80) ,which was significantly higher than 41. 6%( 25 /60) of tumor adjacent tissues ( P < 0. 05) . The expression of survivin was related to clinical stage and lymph node metastasis ( P < 0. 05 ) . Conclusions The detection of the expression of survivin in lung cancer may be a good indicator for metastasis and clinical stage prognosis of lung cancer.