ObjectiveTo investigate the correlation between the expression level of hsa_circ_0105035 in peripheral blood and the occurrence of acute ischemic stroke (AIS), as well as its correlation with cerebral infarction volume and clinical detection indicators. MethodsA total of 310 AIS patients were enrolled as the case group, while another 310 age-matched community people without a history of cardiovascular and cerebrovascular diseases such as stroke or coronary heart disease during the same period was selected as the control group. The expression levels of hsa_circ_0105035 in peripheral blood of all research subjects were detected by quantitative real-time PCR (qRT-PCR), its circular structure was verified by Sanger sequencing and linear digestive enzyme (RNase-R) experiment, and its subcellular location was clarified by nucleocytoplasmic fraction experiment. The cerebral infarction volume of AIS patients was calculated by the Image J software. ResultsHsa_circ_0105035 was mainly distributed in cytoplasm. The expression level of hsa_circ_0105035 in peripheral blood of the case group was lower than that of the control group, with statistically significant difference (P＜0.05). The expression level of hsa_circ_0105035 in peripheral blood of AIS patients was negatively correlated with cerebral infarction volume (rs=-0.127, P=0.045). The platelet volume distribution width and lactate dehydrogenase content of AIS patients in the hsa_circ_0105035 low expression group were larger/higher than those in the hsa_circ_0105035 high expression group, with statistically significant differences (all P＜0.05). ConclusionThe expression of hsa_circ_0105035 in peripheral blood may be correlated with the occurrence of AIS and the cerebral infarction volume.

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