ObjectiveTo investigate the correlation between GPCR116 gene and the occurrence and development of lung adenocarcinoma and its mechanism by bioinformatics analysis. MethodsThe GSE68465 data set (including mRNA data and basic clinical data from 442 lung adenocarcinoma tissue samples and 20 normal tissue samples) was downloaded from the GEO database. To analyze the difference of GPCR116 gene expression between normal tissues and lung adenocarcinoma tissues, as well as the influence of GPCR116 gene expression on the prognosis of lung adenocarcinoma patients and its correlation with clinical features. The gene set enrichment analysis (GSEA) was used to predict the signaling pathways in which the GPCR116 gene participates in lung adenocarcinoma. The differentially expressed genes (DEGs) were screened for gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and the correlation between GPCR116 gene and 40 DEGs with the most significant up-regulation and down-regulation was analyzed. PPI network was constructed to analyze the central node proteins of PPI network and select the key genes of lung adenocarcinoma. Results(1) The expression of GPCR116 gene in lung adenocarcinoma tissues was significantly higher than that in normal tissues. GPCR116 expression was found to be an independent influencing factor for the prognosis of lung adenocarcinoma patients. The high GPCR116 expression group had a better prognosis, and GPCR116 gene expression was correlated with T stage, N stage and smoking history (all P＜0.05). (2) The high GPCR116 gene expression group was mainly enriched in lysosomes, while the low expression group was mainly enriched in signaling pathways such as cell cycle, P53 signaling pathway, mismatch repair, and oocyte meiosis. (3) A total of 235 DEGs were screened out, including 103 down-regulated genes and 132 up-regulated genes. The results of GO enrichment analysis showed that the biological processes of DEGs were mainly involved in nuclear division, organelle fission, and mitotic cell cycle phase transformation, etc. And the results of KEGG pathway enrichment analysis showed that DEGs were mainly enriched in signaling pathways such as human T cell leukemia virus 1 infection, cell cycle, and rheumatoid arthritis, etc. (4) The expression of GPCR116 was positively correlated with that of 13 significantly differential genes, and negatively correlated with that of 20 significantly differential genes. A total of 10 key genes were screened out by PPI network analysis (NKX2-1、SFTPB、SFTPC、SCGB1A1、PITX1、FOXM1、MYBL2、CDCA3、TFF1、WIF1). ConclusionGPCR116 gene is significantly highly expressed in lung adenocarcinoma tissues, and its expression is related to the prognosis of patients with lung adenocarcinoma and can be used as a predictor for prognosis of lung adenocarcinoma patients. The P53 signaling pathway, mismatch repair and lysosomal protein play important roles in the occurrence and development of lung adenocarcinoma by regulating the expression of GPCR116 gene, and its mechanisms may be related to the 10 key genes screened out.

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