ObjectiveTo explore the regulatory mechanism of circular RNAs (circRNAs) in postoperative arterial fibrillation (POAF) after coronary artery bypass grafting (CABG). MethodsThe circRNA expression profile was obtained from data set gene sample expression 97455 (GSE97455) of the Gene Expression Omnibus (GEO) database, and the differential expression of the circRNAs (DECs) in the data set GSE97455 were online analyzed employing the GEO2R software. Then the relevant probe names were transferred into international standard circRNA names; furthermore, target miRNAs and downstream mRNA of circRNAs were obtained using various bioinformatics methods. The most relevant circRNAs, miRNA and downstream target genes were screened, and the screening results were verified by RT-PCR trial. Finally, the figure of circRNA-miRNA-hub gene regulatory network was constructed and the analysis was visualized by Cytoscape 3.7.2. ResultsA total of 55 DECs were obtained, among which 21 circRNAs revealed up-expression and 34 circRNAs with down-expression. After deep-going analysis, the figure of circRNA-miRNA-hub gene network consisted of 6 DECs, 5 miRNAs and 10 hub genes was finally obtained. ConclusionThe research provides a new perspective to reveal the effect of circRNA and circRNA-miRNA-mRNA regulatory network in the POAF, and is preliminarily verified by RT-PCR trial.

无