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目的探讨基质衍生因子-1(SDF-1)对脐带血(UCB)CD34+细胞增殖及归巢相关功能影响。方法采用流式细胞仪测定、造血祖细胞培养、结晶紫染色测定、Transwell实验等方法。结果①SDF-1在体外能通过抑制UCB CD34+细胞凋亡增强细胞生存能力;②与其他造血因子协同SDF-1可在一定程度上促进粒系祖细胞增殖;③SDF-1能提高CD34+细胞的黏附能力和迁移能力。结论 SDF-1能够应用于UCB CD34+细胞体外培养,增强UCB CD34+细胞的归巢相关功能。
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基质衍生因子-1对脐带血CD34~+细胞增殖及归巢相关功能影响的研究
Effects of SDF-1 on the proliferation and homing-related function of UCB CD34 + cells
微创医学 201302期 页码：124-126+142
作者机构： 广西医科大学第一附属医院血液内科
基金信息： 收稿日期: 2013-01-02
基金项目: 国家自然科学基金项目( 合同号: 81160075)
- 中文简介
- 英文简介
- 参考文献
Objective To study effects of stromal derived factor-1 ( SDF-1) on the proliferation and homing-related function of umbilical cord blood ( UCB) CD34cells． Methods Flow cytometry，hematopoietic progenitor cell colony culture，crystal violet staining，and Transwell test were applied． Results ( 1) SDF-1 could promote survival of UCB CD34 + cells by counteracting apoptosis． ( 2) SDF-1 in some degree could promote granulocyte progenitor cell proliferation in synergy with other hematopoietic growth factors． ( 3) SDF-1 could increase the adherent ability and migration ability of CD34 + cells． Conclusion SDF-1 can increase the homing-related ability of UCB CD34 + cells，suggesting that SDF-1 maybe helpful for the in vitro culture of UCB CD34 + cell．
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