ObjectiveTo investigate the primary culture method of human endometrial stromal cells and to compare the morphology and biological behaviors between ectopic endometrial stromal cells (ESC) and normal endometrium stromal cells(NESC), thereby to provide a reliable basis for the establishment of endometriosis (EMS) cell model. MethodsThe improved primary culture method for human endometrial stromal cells was performed. The type of the cells was identified by immunocytochemistry. The difference in morphology was observed under optical microscope.The proliferation and apoptosis of cells were detected by CCK-8 assay and flow cytometry respectively. ResultsThe successful isolation and culture rates of the NESC and ESC were 93.33% and 86.67%, respectively. There were significant differences in the growth rate,morphology and biological behavior between the two kinds of stromal cells. The proliferation of ESC was more obvious than that of the NESC. The relative proliferation rates at 48h and 72h of ESC were higher than those of NESC(P48h＜0.001；P72h＜0.001). The apoptosis rates at 24h, 48h and 96h of ESC were higher than those of NESC(P＜0.001), but there was no significant difference in the apoptosis rate at 72h between NESC and ESC(P＞0.05). ConclusionThe improved primary culture method can increase the success rate of endometrial stromal cell culture. In the cell model in vitro, the growth characteristics of ESC are consistent with the characteristics of EMS. So it can be an ideal model to study the cell mechanism of diseases.

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