微创医学

目的探讨外泌体（EXOs）在胃癌细胞间耐药信息传递的作用。方法选用胃癌敏感细胞株（SGC-7901）及其阿霉素耐药株（SGC-7901/VCR）为模型，用CCK-8绘制细胞生长曲线，计算细胞倍增时间；用超速离心法提取SGC-7901/VCR外泌体（VCR/EXO）；用透射电子显微镜观察鉴定细胞外泌体形态；运用CCK-8法检测VCR/EXO与SGC-7901细胞共培养后对长春新碱(VCR)的IC50的影响。结果SGC-7901、SGC-7901/VCR和SGC-7901/VCR/EXO细胞倍增时间分别是（31.54±1.37）h、（47.21±1.65）h和（42.89±1.73）h；透射电子显微镜下，外泌体呈现典型的圆形或椭圆形杯口状结构，直径为40～100 nm；与VCR/EXO共培养后敏感细胞株SGC-7901对长春新碱的IC50耐药性升高2.29倍，差异有统计学意义（P＜0.05）。结论胃癌外泌体可能具有传递耐药信息的作用，耐药细胞外泌体能够使敏感细胞获得一定的耐药性。虽然外泌体通过细胞间通讯的具体机制仍不清楚，但抑制外泌体的形成和释放，可能会为胃癌的治疗提供新的策略。

ObjectiveTo explore the role of exosomes (EXOs) in the transmission of drug resistance among gastric cancer cells. MethodsSensitive and doxorubicin-resistant gastric cancer cell lines (SGC-7901 and SGC-7901/VCR respectively) were selected as models. Cell growth curves were plotted by CCK-8 assay,then the cell doubling time was calculated. The EXOs of SGC-7901/VCR cells (VCR/EXO) were extracted by ultracentrifugation. Transmission electron microscope was used to identify the morphology of the EXOs. CCK-8 assay was used to assess the effect of co-culture of VCR/EXO and SGC-7901cells on the IC50 of vincristine. ResultsThe doubling time of SGC-7901, SGC-7901/VCR and SGC-7901/VCR/EXO cells were (31.54±1.37) h, (47.21±1.65) h and (42.89±1.73) h respectively. Exosomes with typical round or oval cup shaped structure (diameter was 40-100nm) were observed under transmission electron microscopy.The IC50 of vincristine in SGC-7901 increased 3.29 times after being co-cultured with VCR/EXO(P＜0.05). ConclusionEXOs of gastric cancer cells probably play a role in the transmission. Sensitive cells can obtain drug resistance in a certain degree due to the EXOs of drug-resistant cells. Although the detailed mechanisms of EXOs signaling among cells are still unclear, the inhibition of EXOs formation and release may provide new strategies for the treatment of gastric cancer.