ObjectiveTo investigate the effect of antimacrophage migration inhibitory factor monoclonal antibody on NFκB p65、IκBα expression in mice with ulcerative colitis. MethodsAcute ulcerative colitis was induced by 5% dextran sulfate sodium(DSS). Then the protective effect of antiMIF Ab on ulcerative colotis in mice was observed. Forty mice were randomly distributed into normal control group, UC model group, antiMIF Ab intervention group and saline group.During modeling and the period of administration, to observe score Disease Activity and general situations Index(DAI) of mice, after 8 days killing all mice,performing the gross damage score in colon, observing the colonic histopathological changes under the light microscope on HE staining.The expression of NFκB p65、IκBα protein was detected by immunohistochemical staining. ResultsCompared with normal control mice, the level of NFκB p65 protein increased significantly in colon tissue of mice with UC,the level of IκBα slightly increased. After treatment with antiMIF Ab , the expression level of NFκB p65 significantly lower than that in UC model group,but the expression level of IκBα increased. ConclusionsThese findings suggest that antiMIF Ab had a protective effect on DSSinduced ulcerative colitis in mice downregulating NFκB p65 protein expression. Thus to inhibit the NFκB signaling pathway, excessive activation in the colon tissue of UC mice.

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