微创医学

目的检测肺癌患者血浆Wnt抑制因子-1(WIF-1)基因启动子的甲基化情况并分析其在肺癌早期诊断中的意义。方法应用巢式甲基化特异性PCR（nMSP）法，首先检测肺癌患者血清癌胚抗原(CEA)水平，再分别检测78例肺癌患者、40例肺良性病变患者和40例健康志愿者血浆中WIF-1基因启动子区甲基化状态。结果肺癌患者血清癌胚抗原阳性率为35.90%(28/78)，大致接近血浆标本中WIF-1基因启动子区甲基化率34.62% (27/78)。肺良性病变患者血浆中WIF-1基因启动子区甲基化率为2.50%(1/40),健康志愿者血浆中WIF-1基因启动子区未检测出甲基化。肺癌患者血浆WIF-1基因启动子甲基化和血清癌胚抗原二项联合检测的灵敏度为(62.82%)，特异度为(90.00%)。不吸烟者WIF-1基因启动子甲基化率低于吸烟者。结论外周血浆中WIF-1基因启动子甲基化有成为肺癌的新的检测手段的可能性，并且对肺癌的初步筛查具有一定的临床意义。

ObjectiveTo detect the status of Wnt inhibitory factor-1（WIF-1）promoter methylation in plasma of lung cancer patients and to evaluate its implications in the early diagnosis of lung cancer. MethodsThe nested methylation specific PCR（nMSP）was used to detect the level of carcinoembryonic antigen(CEA) in the serum of patients with lung cancer，then the status of WIF-1 promoter methylation in the plasma of 78 patients with lung cancer，40 patients with benign lung disease and 40 healthy volunteers was detected. ResultsThe positive rate of CEA was 35.90%(28/78),was similar to 34.61% (27/78) of plasma WIF-1 genes promoter methylation in lung cancer patients. The positive rate of WIF-1 gene methylation in plasma of benign lung disease patients was 2.50%(1/40), The WIF-1 genes methylation in plasma was not discovered in healthy volunteers. The sensitivity of combined detection of plasma WIF-1 gene promoter methylation and serum CEA in patients with lung cancer was 62.82%, and the specificity was 90.00%. The WIF-1 gene promoter methylation rate was lower in smokers than that in non-smokers. ConclusionPlasma WIF-1 genes promoter methylation has the possibility of becoming a new tumor marker in lung cancer. The detection of WIF-1 genes promoter methylation has a certain potential value for the early diagnosis of lung cancer.