微创医学

目的探讨HLA-DRB1等位基因多态性对广西青年女性宫颈癌发生的影响，为寻找广西女性宫颈癌的遗传易感基因或拮抗基因提供线索。方法选取广西地区25～35岁确诊为宫颈癌的患者（宫颈癌组）、无癌健康年轻女性（对照组）各57例作为研究对象，采集研究对象外周血并提取基因组DNA，应用PCR-SSP方法对HLA-DRB1等位基因进行基因型检测，最后进行统计学分析。结果宫颈癌组HLA-DRB1*04/10等位基因出现频率显著高于对照组，差异有统计学意义（P＜0.05）；宫颈癌组HLA-DRB1*09等位基因出现频率显著低于对照组，差异有统计学意义（P＜0.05）；而两组间HLA-DRB1*01/07/08/11/12/13/14/15/16/17/18等位基因出现频率比较，差异无统计学意义（P＞0.05）。结论HLA-DRB1*04/10等位基因可能是广西青年女性宫颈癌发生的易感基因；HLA-DRB1*09等位基因可能是广西青年女性宫颈癌的保护基因。而HLA-DRB1*01/07/08/11/12/13/14/15/16/17/18等位基因可能与广西青年女性宫颈癌遗传易感性无关。

ObjectiveStudy on the relationship of Guangxi young women suffering from cervical cancer with HLA-DRB1 allele polymorphism. Provide clues for seeking hereditary susceptibility gene or resistant gene of cervical cancer of Guangxi women. MethodsChoose the cervical cancer diagnosed female patient and health woman 57 cases respectively aged between 25 and 35 of Guangxi as subject investigated. Take their peripheral blood samples to extract genome DNA. Then detect HLA-DRB1 genetype applying PCR-SSP technology. Finally the data were statistically analyzed. ResultsThe allele carrying rate of HLA-DRB1*04/10 in the cervical cancer group was higher than the health control group with statistically significant difference(P＜0.05). The allele carrying rate of HLA-DRB1*09 in the cervical cancer group was lower than the health control group with statistically significant difference(P＜0.05). There was no significant difference of the allele carrying rate of HLA-DRB1*01/07/08/11/12/13/14/15/16/17/18 between two groups (P＞0.05). ConclusionsHLA-DRB1*04/10 alleles are probably the susceptibility genes of cervical cancer of Guangxi young women；HLA-DRB1*09 alleles are probably the protective genes of cervical cancer of Guangxi young women；HLA-DRB1*01/07/08/11/12/13/14/15/16/17/18 alleles seem irrelevant to hereditary susceptibility of cervical cancer of Guangxi young women.