ObjectiveStudy on the methods and techniques of long term beating heart preservation. MethodSelected 6 adult Guangxi Bama miniature pigs, after the success of anesthesia, took the chest of the median incision, whole body heparinization, didn’t cut pericardium. First in the brachiocephalic trunk perfusion tube was inserted, and then the superior vena cava and the left inferior pulmonary vein were cut off, and then left ventricle was emptied with hand, and then the heart was perfused with cardioplegic solution. The heart was connected to the perfusion device after it was removed, and was perfused with diluted blood which white blood cells and platelets have been removed and beating recovery (non-working heart model). ResultsThe 6 isolated hearts were successfully preservated in a beating state for 8h. During preservation, perfusion pressure was not rising. ConclusionPreservation isolated heart in a beating state is feasible. Preservation of key technologies is to reduce the destruction of red blood cells, maintain appropriate perfusion pressure, the effective decompression of the left ventricle and to prevent the loss of heart water.

无