微创医学

目的探讨聚桂醇原液在组织中的病理变化过程和硬化剂的病理机制。方法取标准健康成年日本大耳白兔，在兔胃壁组织中分别注入1%（F组）、3%（H组）聚桂醇原液0.5 mL，根据试验设定的时间，在标注的胃壁组织上取出注射过的全层胃壁组织，经固定、切片、染色后，显微镜下观察其成纤维细胞及胶原纤维的变化，找到组织纤维硬化的基本病理变化过程。结果F组在第5天开始可见浆膜有炎症反应并有着色较深的成纤维细胞增生；成纤维细胞数量第6天达高峰，以后逐渐减少，同时纤维细胞逐渐增多，胶原纤维也逐渐增多；第12天大量形成胶原纤维。H组在第3天黏膜下有少量成纤维细胞增生，到第4天达最高峰，以后逐渐减少，同时胶原纤维逐渐增多；第8天以后，成纤维细胞逐渐减少，变成纤维细胞，胶原纤维平稳增加。结论聚桂醇液在兔胃壁组织中可以产生明显的炎性反应过程，并最终产生大量的胶原物质，形成组织纤维化；这个过程与药物浓度无关；药物浓度与在组织中产生成纤维细胞的时间有关。

ObjectiveTo explore the pathological change of Lauromactogol injection in tissues. MethodsEighty Japanese white rabbits were recruited in our study and injected with 1%(F group) and 3%(H group) Lauromacrogol injection 0.5 ml in their gastric wall. Then the gastric walls were excised after different predetermined time. Changes of fibrocyte and collagen fiber were observed by optical microscope after fixation,section and staining. ResultsInflammatory reaction and fibroblast hyperplasia were observed in F group at the fifth day after injected and the number of fibroblast reached the peak at the sixth day. The numbers of fibroblast gradually reduced,at the same time the collagen fiber gradually grew in number. And the fibroblast reduced and collagen fiber stably grew. ConclusionsThe significant inflammatory reaction produce in gastric wall tissue of rabbit and abundant collagen fiber and form fibrosis in the tissue. But this process have nothing to do with drug concentration, and are related to time.