Objective To assess the germicidal efficacy of selfmade disinfection tank with ultraviolet LED. Methods A total of 100 sterile forceps/tweezers were artificially contaminated with 5 standard bacterial strains (Staphylococcus aureus, Escherichia coli, ESBLsproducing Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis), and were divided into experimental group (group A, n=50) and control group (group B, n=50). The forceps/tweezers of group A were sterilized using selfmade disinfection tank with ultraviolet LED, and the forceps/tweezers of group B were sterilized using traditional storage tank with pressuresteam sterilization. The germicidal efficacy for 5 standard bacterial strains was evaluated in the two approaches. Results In group A, 47 samples was successful in sterilization and 3 samples failed to be sterilized. The effective rate of group A was 94%. In group B, 48 samples was successful in sterilization and 2 samples failed to be sterilized. The effective rate of group B was 96%. There was no difference in the sum of sterilized samples between the two groups (P＞0.05). In group A, the sterilization rate of Escherichia coli, ESBLsproducing Escherichia coli and Pseudomonas aeruginosa was 100%, and the sterilization rate of Staphylococcus aureus and Enterococcus faecalis was 90%. In the group B, the sterilization rate of Staphylococcus aureus, Escherichia coli and Enterococcus faecalis was 100%, and the sterilization rate of ESBLsproducing Escherichia coli and Pseudomonas aeruginosa was 100%. There was no difference in the category of sterilization between the two groups (P＞0.05). Conclusions The selfmade disinfection tank with 200 to 275 nm wave length of ultraviolet LED has the same germicidal efficacy as the traditional disinfection storage tank with high pressure. This approach has certain feasibility and is worthy of further research.

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