微创医学

目的探讨基质金属蛋白酶2（MMP2）及基质金属蛋白酶抑制剂（TIMP2）在结肠腺瘤、结肠癌及正常结肠黏膜组织中的表达及其意义。方法采用免疫组化法检测MMP2及TIMP2在正常大肠黏膜、结肠腺瘤及腺瘤癌变组织中的表达情况。结果 MMP2及TIMP2在正常结肠黏膜、结肠腺瘤组织及结直肠癌组织中均有表达，其中结直肠癌组织MMP2的表达量明显高于正常组织及腺瘤组织，差异有统计学意义（P＜0.05），腺瘤性息肉中高级别上皮内瘤变组的MMP2表达量高于低级别组（P＜0.05）。TIMP2在腺瘤组织与正常组织中的表达无差别，在结肠腺癌组织中的表达较正常组织中降低（P＜0.05）。结论 MMP2及TIMP2在结肠腺瘤癌变过程中分别呈高表达、低表达，二者表达的失衡在正常黏膜-腺瘤-癌的病理形成过程中起一定作用。

Objective To investigate the expression and significance of matrix metalloproteinases 2 (MMP2) and tissue inhibitor of metalloprotease 2(TIMP2) in colonic adenoma, colonic carcinoma and normal colonic mucosa tissues. Methods The expressions of MMP2 and TIMP2 were measured using immunohistochemical staining in normal colonic mucosa, colonic adenoma and carcinoma tissues. Results MMP2 and TIMP2 were expressed in normal colonic mucosa, colonic adenoma and colorectal carcinoma tissues. The expression of MMP2 in colorectal carcinoma tissue was significantly higher than that in normal tissue and adenoma tissue (P＜0.05). For the adenomatous polyps with intraepithelial neoplasia, MMP2 expression in the high grade group was higher than that in the low grade group (P＜0.05). TIMP2 expression in adenoma tissue had no difference with that in normal tissue, whereas it is lower in colonic adenocarcinoma tissue compared with normal tissue (P＜0.05). Conclusion The expression levels of MMP2 and TIMP2 in the process of colonic adenoma canceration are increased and decreased respectively. And this imbalance may play a role in the progression from normal mucosa to adenoma and to cancer.