Objective To systematically evaluate the effect of comprehensive insulation nursing measures on intraoperative body temperature and clinical indicators in patients. Method CNKI, Wanfang Data Knowledge Service Platform, VIP Database, PubMed, EMbase, Cochrane Library and other databases were searched to include randomized controlled trials of intraoperative insulation nursing measures. After data extraction and literature quality evaluation, RevMan 5.4 software was used to conduct a Meta-analysis of the literature that met the criteria. Result A total of 10 articles involving 1 035 paitients were included. The results of Meta-analysis showed that the intraoperative body temperature of the experimental group (treated with comprehensive insulation nursing measures) was significantly higher than that of the control group (treated with routine nursing insulation measures), the incidence of postoperative hypothermia and shivering was lower than that in the control group, and the postoperative epinephrine and norepinephrine levels were lower than those in the control group (all P<0.05). However, the postoperative levels of C-reactive protein in the two groups were similar (P>0.05). Conclusion Compared with routine nursing insulation measures, comprehensive insulation nursing measures can improve the intraoperative temperature at different periods, prevent the occurrence of intraoperative hypothermia and shivering, and maintain the stress indicator level.