Objective To compare the application effect of robot-assisted percutaneous pedicle screw placement and conventional open pedicle screw placement in fracture of lumbar vertebrae. Methods A total of 111 patients with fracture of lumbar vertebrae who received pedicle screw placement were included as the study objects. Fifty-two patients who received robot-assisted percutaneous pedicle screw fixation were assigned as study group, and 49 patients who received conventional open pedicle screw fixation at the same time were assigned as control group.The accuracy of screw placement, operation time, intraoperative blood loss, postoperative hospital stay, Visual Analogue Scale (VAS) score, Neck Disability Index(NDI) score, occurrence of postoperative complications, fracture healing time of injured vertebra, Cobb angle of sagittal plane of injured vertebra were compared. Results Compared with the control group, the accuracy of screw placement was higher, the intraoperative blood loss was less, and the fracture healing time of injured vertebra was shorter in the study group (all P<0.05). There was no statistically significant difference in operation time, postoperative hospital stay and complication rate between the two groups (all P>0.05). There was no statistically significant difference in the Cobb angle of sagittal plane of injured vertebra and the percentage of anterior height of injured vertebra between the two groups before operation, 1 week after operation and at the last follow-up (all P>0.05). However, with the prolongation of time, the Cobb angle of sagittal plane of injured vertebra in the two groups showed a downward trend, while the percentage of anterior height of injured vertebra showed an upward trend (all P<0.05). There was no statistically significant difference in VAS score and NDI score between the two groups before operation and 1 month after operation (all P>0.05), but compared with preoperative, the VAS score and NDI score of the two groups decreased 1 month after operation (all P<0.05). Conclusion In operation of fracture of lumbar vertebra, robot-assisted percutaneous pedicle screw placement is superior to the conventional open pedicle screw placement in terms of the accuracy of screw placement, intraoperative blood loss and fracture healing time, which makes the opertion method safe and feasible.