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目的探讨胚胎实验室采取改善的ICSI操作技巧后,ICSI临床结局的改善状况。方法比较采取改善的操作技巧前后ICSI的受精率、卵裂率、优质胚胎率、临床妊娠率及流产率。结果采取改善的ICSI操作技术后受精率(77.50%vs 86.37%)、卵裂率(95.13%vs 98.00%)、优质胚胎率(55.90%vs 43.69%)变化不明显(P>0.05),但种植率(19.38%vs30.41%)和临床妊娠率(33.77%vs 44.83%)明显提高,流产率明显下降(26.92%vs 10.64%)。结论改善ICSI操作技巧后,临床结局得到明显改善,即种植率及临床妊娠率明显提高,流产率明显降低。
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改善卵胞浆内单精子注射临床结局的实验室操作技巧
Laboratory practice for improving clinical outcome of intracytoplasmic sperm injection
微创医学 201002期 页码：95-97
作者机构： 广西壮族自治区人口和计划生育研究中心； 广西壮族自治区妇幼保健院生殖中心
基金信息： 【基金】 广西区卫生厅科研基金资助项目(Z2007013)
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Objective To explore the improvement on clinical outcome of intracytoplasmic sperm injection(ICSI) with improved laboratory practice.Methods Comparing the fertilization rates,cleavage rates,good quality embryo rates,clinical pregnancy rates and abortion rates before and after implementing ICSI improved laboratory practice.Results After implementing ICSI improved laboratory practice,there was no significant difference between the fertilization rates(77.50% vs 86.37%),the cleavage rates(95.13% vs 98.00%),and good quality embryo rates(55.90% vs 43.69%),but implantation rate(19.38% vs 30.41%) and clinical pregnancy rate(33.77% vs 44.83%) were improved,and the abortion rate(26.92% vs 10.64%) decreased significantly.Conclusion After implementing improved laboratory practice,clinical outcome were improved,with implantation rate and clinical pregnancy rate improved and abortion rate decreased significantly.
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