微创医学

目的在垂体腺瘤组织中检测表皮生长因子受体（EGFR）的表达水平并分析其与临床病理类型、肿瘤增殖情况、侵袭性等的关系,为垂体瘤诊断和治疗提供理论依据和临床指导。方法选取65例垂体腺瘤患者术前血清、15例正常志愿者血清,采用酶联免疫分析技术对其EGFR水平进行检测。结果①EGFR水平在侵袭性组为（11.58±0.72）ng/mL、非侵袭性组为（11.44±0.68）ng/mL,与对照组（11.26±0.87）ng/mL相比有明显差异（P〈0.05）,且侵袭性组明显高于非侵袭性组（P〈0.05）。②EGFR水平在垂体微腺瘤组、垂体大腺瘤组、垂体巨大腺瘤组中分别为（11.36±0.17）ng/mL、（11.45±0.69）ng/mL、（11.67±0.62）ng/mL,与对照组（11.26±0.87）ng/mL相比有明显差异（P〈0.05）;EGFR在垂体腺瘤各组间存在明显差异（P〈0.001）,且与垂体腺瘤大小呈正相关。③EGFR水平在功能性垂体腺瘤组为（11.52±0.52）ng/mL,与非功能性垂体腺瘤组（11.48±0.71）ng/mL比较无明显差异（P〉0.05）,且功能性垂体腺瘤各组中无明显差异。结论术前EGFR的检测可以在一定程度上反映垂体腺瘤的增殖情况,术前EGFR的检测有可能成为诊断垂体腺瘤侵袭性和评估预后的良好生物学指标

物学指标。 Objective To detect the expression level of epidermal growth factor receptor（EGFR） in the pituitary adenoma,and explore its relationship with clinical pathology,proliferation and invasivenss to provide theoretical basis and clinical guideline for the diagnosis and treatment of pituitary adenoma.Methods ELISA was used to determine the levels of EGFR in preoperative serum from 65 patients with pituitary adenomas,and 15 healthy blood donors were used as control.Results 1 The level of serum EGFR in pituitary adenomas was significantly higher than that in healthy control group（11.26±0.87）ng/ml,P0.05）.The level of serum EGFR was significantly higher in invasive pituitary adenomas（11.58±0.72）ng/ml than that in non-invasive ones（11.44±0.68）ng/ml,P0.05）.2.The level of serum EGFR was（11.36±0.17）ng/ml in patients with pituitary microadenoma,（11.45±0.69）ng/ml in patients with pituitary macroadenoma,and（11.67±0.62）ng/ml in patients with pituitary gaint adenoma.The levels of serum EGFR in patients with different kinds of pituitary adenoma were significantly higher than the level in healthy control group（11.26±0.87）ng/ml,P0.05）.Furthermore,significant difference in the level of serum EGFR was observed among patients with different kinds of pituitary adenoma（P0.05）,and the serum level of EGFR was positively correlated to the size of pituitary adenoma.3.The levels of serum EGFR in patients with function and non-function adenoma were（11.52±0.52）ng/ml and（11.48±0.71）ng/mL,respectively,but no significant difference in the level of serum EGFR was observed between them.Conclusions EGFR level in preoperative serum of patient with pituitary adenoma reflexes the proliferative status of pituitary adenoma,and the level of EGFR can be a useful marker in determining invasive behavior of pituitary adenomas